Influence of arbuscular mycorrhizal fungi density on growth and metabolism of Handroanthus serratifolius (Vahl) S.O. Grose seedlings.

Studies on the relationship between Handroanthus serratifolius and arbuscular mycorrhizal fungi (AMF) are limited in the literature. The influence of AMF spore density on plant development is fundamental information to determining the degree of benefits in this relationship. Therefore, the objective of this study was to investigate the effects of different AMF spore densities on thirty-day-old H. serratifolius seedlings, focusing on growth and biochemical parameters using a completely randomized experimental design with three different spore densities and control. The spore densities were classified as low, medium, and high, with 1.54, 3.08, and 12.35 spores g-1 , respectively. Plant growth analysis, mycorrhizal colonization, nitrogen compound concentration, and carbohydrate analysis were performed. The medium spore density treatment showed the greatest increases in biomass, height, leaf area, and root volume. Furthermore, greater absorption of phosphorus and better dynamics in nitrogen metabolism were observed in mycorrhizal plants compared to the control since the ammonium and nitrate compounds were rapidly incorporated into protein and chlorophyll compounds. The carbohydrate analysis revealed the influence of source-sink dynamics on sugar concentration in different plant parts. These findings support the importance of determining the appropriate spore density for assessing the symbiotic relationship between forest species and AMF.

Arbuscular Mycorrhizal Fungi Promote Physiological and Biochemical Advantages in Handroanthus serratifolius Seedlings Submitted to Different Water Deficits.

Climate change causes increasingly longer periods of drought, often causing the death of plants, especially when they are in the early stages of development. Studying the benefits provided by arbuscular mycorrhizal (AM) fungi to plants in different water regimes is an efficient and sustainable strategy to face climate change. Thus, this study investigated the influence of AM fungi on Handroanthus serratifolius seedlings under different water regimes, based on biochemical, and nutritional growth parameters. The experiment was carried out in H. serratifolius seedlings cultivated with mycorrhizas (+AMF) and without mycorrhizas (-AMF) in three water regimes; a severe water deficit (SD), a moderate water deficit (MD), and a well-watered (WW) condition. AM fungi provided greater osmoregulation under water deficit conditions through the accumulation of soluble sugars, total free amino acids, and proline, as well as by reducing sugar. The increase in the absorption of phosphorus and nitrate was observed only in the presence of fungi in the well-watered regimen. A higher percentage of colonization was found in plants submitted to the well-watered regimen. Ultimately, AM fungi promoted biochemical, nutritional, and growth benefits for H. serratifolius seedlings under the water deficit and well-hydrated conditions, proving that AMF can be used to increase the tolerance of H. serratifolius plants, and help them to survive climate change.

Seasonality and Phosphate Fertilization in Carbohydrates Storage: Carapa guianensis Aubl. Seedlings Responses.

The low availability of phosphorus and water in soil can promote the remobilization of carbohydrates in the plant, releasing energy to mitigate stress. In this context, our objective was to analyze the production and allocation of carbohydrates in plants of Carapa guianensis Aubl. submitted to different doses of phosphate fertilization, during the rainy and dry seasons, in the western region of Pará. We used three phosphorus dosages (0, 50, 250 kg ha−1) as treatments. We evaluated the plants during the dry and wet seasons. We quantified dry matter production, phosphorus content, total soluble sugars, reducing sugars, sucrose, and starch. Phosphate fertilization and different evaluation periods influenced carbohydrate concentrations (p < 0.05) in plants. The highest levels of P in the leaves were registered in October and, in the roots the content decreased with the passage of time in all treatments. The control had higher dry matter production in leaves and stems. During the dry season, there was an accumulation of carbohydrates in plants and a low production of dry matter. Soluble sugars and sucrose tended to be allocated to the stem, reducing sugars to the leaves and starch to the roots, in most periods. In general, C. guianensis seedlings were not very responsive to phosphorus addition.

Analysis of the maxillary dental arch after rapid maxillary expansion in patients with unilateral complete cleft lip and palate.

INTRODUCTION: Although rapid maxillary expansion has been extensively studied in noncleft patients, scarce evidence of maxillary effects is found for patients with oral clefts. Our objective was to evaluate the dentoalveolar effects of rapid maxillary expansion in children with unilateral complete cleft lip and palate compared with noncleft patients. METHODS: The experimental group included 25 patients with unilateral complete cleft lip and palate and posterior crossbite (9 girls, 16 boys; mean age, 10.6 years). The control group comprised 27 noncleft patients with posterior crossbite (14 boys, 13 girls; mean age, 9.1 years). The experimental group had lip and palate repairs at 3 and 12 months of age, respectively, and maxillary expansion was indicated before a secondary alveolar bone graft. Digital dental models were obtained immediately before expansion and 6 months after expansion with a laser scanner. Canine and posterior tooth inclinations, transversal widths, arch perimeters, arch lengths, palatal depths, and palatal volumes were digitally measured. The paired t test was used for interphase comparisons, and the independent t test was used for intergroup comparisons (P <0.05). RESULTS: In the experimental group, expansion produced buccal tipping of the canines and posterior teeth and significant increases of all maxillary transverse measurements, arch perimeter, palatal volumes, and palatal depths; only arch lengths decreased. No differences were observed between the groups for any measurement, except for intermolar distance. CONCLUSIONS: Rapid maxillary expansion produced similar changes in the patients with unilateral complete cleft lip and palate compared with the noncleft patients except for arch length and palatal depth that decreased after expansion in the children with oral clefts.

Efeito transversal da placa labioativa aberta associada à extensão lingual – estudo em modelos digitais / Transverse effect of the expanded lip bumper associated to the lingual extension – a digital model study

O objetivo do presente estudo clínico foi avaliar as dimensões transversais do arco dentário inferior com o uso da placa labioativa aberta e fixa associada à extensão lingual de canino decíduo a primeiro molar permanente. Material e métodos: a amostra constou de 14 pacientes no estágio de dentadura mista e com idade média de oito anos e seis meses. Todos os pacientes realizaram expansão rápida da maxila e expansão lenta do arco inferior com a placa labioativa. Modelos de gesso foram obtidos nas fases de pré-tratamento e pós-tratamento, e digitalizados com o auxílio do scanner 3Shape R700 3D (3Shape A/S, Copenhagen, Dinamarca). Medições transversais foram realizadas para as distâncias entre as pontas de cúspide dos caninos decíduos, pontas de cúspide mesiovestibulares dos molares decíduos e primeiro molar permanente, e ponto médio cervical da face vestibular. O teste t foi utilizado para determinar se o aumento nas larguras transversais foi significativo. Resultados: observou-se um aumento transversal estatisticamente signifi cativo (p < 0,05), que variou de 4,11 mm a 4,51 mm nos referenciais utilizados no nível das pontas de cúspide. Conclusão: a placa labioativa aberta e fixa apresentou um efeito transversal significativo em aproximadamente seis meses de tratamento...

The aim of the current clinical study was to evaluate the transverse dimensions of the lower dental arch with the use of the fixed and expanded lip bumper associated to the lingual extension from the deciduous canine to the permanent first molar. Methods: the sample was comprised of 14 patients in the mixed dentition with a mean age of 8 years and 6 months. All patients were submitted to rapid palatal expansion and dentoalveolar expansion of the lower dental arch with a lip bumper. Pre-treatment and post-treatment cast models were obtained and digitized with the 3Shape R700 3D (3Shape A/S, Copenhagen, Denmark) scanner. Transverse distances between the cusp tips of the canines, the mesial buccal cusps of the deciduous molars and of the permanent first molars and between the cervical middle points of the buccal aspects of these teeth were measured. The t tests were performed to determine whether an increase in transverse distances was signifi cant. Results: a statistically significant transverse increase (p < 0,05) ranging from 4.11 mm to 4.51 mm was observed in the cusp tips. Conclusion: the fixed and expanded lip bumper presented a significant transverse effect within approximately 6 months of treatment...

Transverse effect of Haas and Hyrax appliances on the upper dental arch in patients with unilateral complete cleft lip and palate: a comparative study.

OBJECTIVE: The aim of the present study was to evaluate the transverse effect of rapid maxillary expansion in patients with unilateral complete cleft lip and palate while comparing the Haas and Hyrax appliances. METHODS: The sample consisted of 48 patients divided into two groups: Group I = 25 patients treated with modified Haas appliance (mean age: 10 years and 8 months); and Group II = 23 patients treated with Hyrax appliance (mean age: 10 years and 6 months). Cast models were taken during pre-expansion and after removal of the appliance, at the end of the retention period. The models were scanned with the aid of the 3 Shape R700 3D scanner. Initial and final transverse distances were measured at cusp tips and cervical-palatal points of maxillary teeth by using the Ortho Analyzer 3D software. RESULTS: The mean expansion obtained between cusp tips and between cervical-palatal points was, respectively: for inter-canine width, 4.80 mm and 4.35 mm with the Haas appliance and 5.91 mm and 5.91 mm with the Hyrax appliance; as for first deciduous molars or first deciduous premolars, the values obtained were 6.46 mm and 5.90 mm in the Haas group, and 7.11 mm and 6.65 mm in the Hyrax group; with regard to first molars, values were 6.11 mm and 5.24 mm in the Haas group, and 7.55 mm and 6.31 mm in the Hyrax group. CONCLUSION: Rapid maxillary expansion significantly increased the transverse dimensions of the upper dental arch in patients with cleft palate, with no significant differences between the Hass and Hyrax expanders.

Transverse effect of Haas and Hyrax appliances on the upper dental arch in patients with unilateral complete cleft lip and palate: A comparative study

Objective: The aim of the present study was to evaluate the transverse effect of rapid maxillary expansion in patients with unilateral complete cleft lip and palate while comparing the Haas and Hyrax appliances. Methods: The sample consisted of 48 patients divided into two groups: Group I - 25 patients treated with modified Haas appliance (mean age: 10 years 8 months); and Group II - 23 patients treated with Hyrax appliance (mean age: 10 years 6 months). Casts were taken during pre-expansion and after removal of the appliance at the end of the retention period. The models were scanned with the aid of the 3 Shape R700 3D scanner. Initial and final transverse distances were measured at cusp tips and cervical-palatal points of maxillary teeth by using the Ortho Analyzer(tm) 3D software. Results: The mean expansion obtained between cusp tips and cervical-palatal points for inter-canine width was 4.80 mm and 4.35 mm with the Haas appliance and 5.91 mm and 5.91 mm with the Hyrax appliance. As for first premolars or first deciduous molars, the values obtained were 6.46 mm and 5.90 mm in the Haas group and 7.11 mm and 6.65 mm in the Hyrax group. With regard to first molars, values were 6.11 mm and 5.24 mm in the Haas group and 7.55 mm and 6.31 mm in the Hyrax group. Conclusion: Rapid maxillary expansion significantly increased the transverse dimensions of the upper dental arch in patients with cleft palate, with no significant differences between the Hass and Hyrax expanders. .

Objetivo: avaliar o efeito transversal na arcada dentária superior do procedimento de expansão rápida da maxila em pacientes com fissura transforame incisivo Aunilateral, comparando os expansores tipo Haas modificado e de Hyrax. Métodos: a amostra constou de 48 pacientes divididos em dois grupos: grupo I, 25 pacientes que utilizaram o aparelho expansor tipo Haas modificado, com média de idade de 10 anos e 8 meses; e grupo II, 23 pacientes que utilizaram o Hyrax, com média de idade de 10 anos e 6 meses. Modelos de gesso foram realizados na fase pré-expansão e após 6 meses de contenção, após a remoção do aparelho. Os modelos foram digitalizados com auxílio do scanner 3Shape R700 3D e as distâncias transversais iniciais e finais foram medidas entre as pontas de cúspides e pontos cervicopalatinos de dentes superiores pelo método digital no software OrthoAnalyserT 3D. Resultados: a média de expansão obtida entre as pontas de cúspides e entre os pontos cervicopalatinos, respectivamente, para a distância intercaninos, foi de 4,80mm e 4,35mm para o Haas e de 5,91mm e 5,91mm para o Hyrax; 6,46mm e 5,90mm para os primeiros molares decíduos ou primeiros pré-molares no grupo Haas, 7,11mm e 6,65mm no grupo Hyrax; e 6,11mm e 5,24mm para os primeiros molares no grupo Haas e 7,55mm e 6,31mm no grupo Hyrax. Conclusão: o procedimento de expansão rápida da maxila produziu aumentos significativos das dimensões transversais da arcada dentária superior em pacientes com fissura, sem diferenças significativas entre os expansores Haas modificado e Hyrax. .

Prevalence of mesiodens in orthodontic patients with deciduous and mixed dentition and its association with other dental anomalies.

OBJECTIVE: To determine the prevalence of mesiodens in deciduous and mixed dentitions and its association with other dental anomalies. MATERIAL AND METHODS: Panoramic radiographs of 1,995 orthodontic patients were analyzed retrospectively, obtaining a final sample of 30 patients with mesiodens. The following aspects were analyzed: gender; number of mesiodens; proportion between erupted and non-erupted mesiodens; initial position of the supernumerary tooth; related complications; treatment plan accomplished; and associated dental anomalies. The frequency of dental anomalies in the sample was compared to reference values for the general population using the chi-square test, with a significance level set at 5%. RESULTS: The prevalence of mesiodens was 1.5% more common among males (1.5:1). Most of the mesiodens were non-erupted (75%) and in a vertical position, facing the oral cavity. Extraction of the mesiodens was the most common treatment. The main complications associated with mesiodens were: delayed eruption of permanent incisors (34.28%) and midline diastema (28.57%). From all the dental anomalies analyzed, only the prevalence of maxillary lateral incisor agenesis was higher in comparison to the general population. CONCLUSIONS: There was a low prevalence of mesiodens (1.5%) in deciduous and mixed dentition and the condition was not associated with other dental anomalies, except for the maxillary lateral incisor agenesis.

Prevalence of mesiodens in orthodontic patients with deciduous and mixed dentition and its association with other dental anomalies

OBJECTIVE: To determine the prevalence of mesiodens in deciduous and mixed dentitions and its association with other dental anomalies. MATERIAL AND METHODS: Panoramic radiographs of 1,995 orthodontic patients were analyzed retrospectively, obtaining a final sample of 30 patients with mesiodens. The following aspects were analyzed: gender ; number of mesiodens; proportion between erupted and non-erupted mesiodens; initial position of the supernumerary tooth; related complications; treatment plan accomplished; and associated dental anomalies. The frequency of dental anomalies in the sample was compared to reference values for the general population using the chi-square test (c²), with a significance level set at 5%. RESULTS: The prevalence of mesiodens was 1.5% more common among males (1.5:1). Most of the mesiodens were non-erupted (75%) and in a vertical position, facing the oral cavity. Extraction of the mesiodens was the most common treatment. The main complications associated with mesiodens were: delayed eruption of permanent incisors (34.28%) and midline diastema (28.57%). From all the dental anomalies analyzed, only the prevalence of maxillary lateral incisor agenesis was higher in comparison to the general population. CONCLUSION: There was a low prevalence of mesiodens (1.5%) in deciduous and mixed dentition and the condition was not associated with other dental anomalies, except for the maxillary lateral incisor agenesis.

OBJETIVO: determinar a prevalência de mesiodens nos estágios de dentição decídua e mista, e verificar sua associação com outras anomalias dentárias. MÉTODOS: radiografias panorâmicas de 1.995 pacientes ortodônticos foram analisadas retrospectivamente, obtendo-se uma amostra de 30 pacientes com o mesiodens. Os seguintes aspectos foram analisados: distribuição entre os sexos, número de mesiodens; se irrompido ou não irrompido; posição; complicações; tratamento instituído, e anomalias dentárias associadas. A frequência de anomalias dentárias na amostra estudada foi comparada a valores de referência para a população em geral por meio do teste qui-quadrado (c²), com um nível de significância de 5% (p < 0,05). RESULTADOS: a prevalência de mesiodens foi de 1,5%, sendo mais comum no sexo masculino (1,5:1). A maior parte dos mesiodens estavam não irrompidos (75%) e numa posição vertical, voltada para a cavidade bucal. O tratamento mais empregado foi a exodontia. As principais complicações associadas ao mesiodens foram o atraso na erupção dos incisivos permanentes (34,28%) e diastema mediano (28,57%). Pacientes com mesiodens não apresentaram prevalência aumentada de microdontia, agenesia de dentes permanentes ou outros supranumerários. De todas as anomalias analisadas, apenas a prevalência de agenesia de incisivo lateral superior mostrou-se aumentada em comparação à população em geral. CONCLUSÃO: o mesiodens foi encontrado em uma prevalência baixa (1,5%) nas dentições decídua e mista, e não apresentou associação com outras anomalias dentárias, com exceção da agenesia de incisivo lateral superior.

Prevalência de dentes supranumerários na região dos pré-molares e sua associação com a presença dos terceiros molares / Prevalence of supranumerary teeth on pre-molars region and their association with third molars

O objetivo do presente estudo foi determinar a prevalência de supranumerários na região dos pré-molares nos estágios de dentadura decídua, mista e permanente, além de verificar sua associação com a presença de terceiros molares. Foram avaliadas radiografias panorâmicas de 1.976 pacientes matriculados no curso de Ortodontia Preventiva e Interceptiva da Profis (Sociedade de Promoção Social do Fissurado Lábio-Palatal), em Bauru-SP. As radiografias foram analisadas em negatoscópio, em ambiente escurecido, por um único examinador que determinou a presença de supranumerários na região dos pré-molares, bem como a presença de terceiros molares. A associação entre o aparecimento de supranumerários e a presença de terceiros molares foi verificada pelo Teste de McNemar. O Teste exato de Fisher foi aplicado para verificar o dimorfismo sexual. Constatou-se uma prevalência de 0,45% de supranumerários na região dos pré-molares na amostra estudada, sendo 11% na maxila e 89% na mandíbula, e não foi observado dimorfismo sexual em relação ao aparecimento dos supranumerários (p=0,32). Nas radiografias avaliadas, foi observado que os quatro terceiros molares estavam presentes sempre que verificada a presença dos supranumerários, detectando uma associação estatisticamente significante (p < 0,01) entre o aparecimento dos pré-molares supranumerários e a presença dos terceiros molares. Concluiu-se que a incidência de pré-molares supranumerários foi baixa na população estudada, e que existe associação entre o aparecimento destes supranumerários com a presença dos terceiros molares.

The purpose of the present study was to determine the prevalence of supranumerary teeth on the pre-molars region during the deciduous, mixed and permanent dentition and also verify their association with the presence of third molars. 1.976 panoramic radiographic from patients of the Preventive and Interceptive Orthodontic Course from Profis (Sociedade de Promoção Social do Fissurado Lábio-Palatal), in Bauru-SP were evaluated. The radiographies were analyzed in viewing box in a dark room by one unique examiner who determined the presence of supranumerary teeth on pre-molars region and also the presence of third molars. The association between the supranumerary and the third molars was verified by the McNemar test. The Fisher’s exact test was used to verify the sexual dimorphism. A prevalence of 0,45% of supranumerary teeth on the pre-molars region was found on the sample, 11% on the maxilla and 89% on the mandible. No sexual dimorphism was found (p=0,32). A statistically significant difference (p < 0,01) association between supranumerary pre-molars and the presence of third molar was found. The conclusion is that the incidence of supranumerary pre-molars is low on the examined sample and there is an association between the supranumerary pre-molars and the presence of third molars.

Morphological characterization of fruits, diaspores and germination of Miconia ligustroides (DC) Naundim (Melastomataceae) / Caracterização morfológica dos frutos, diásporos e germinação de Miconia ligustroides (DC) Naundim (Melastomataceae)

Miconia ligustroides (DC.) Naudim, popularly known as "vassoura-preta" or "jacatirão-do-brejo" is widely distributed in Brazil. It is a recommended species for restoration of riparian areas and its fruits are attractive to wildlife. However, little is known about the morphology of its fruits and diaspores. Such morphological characterization studies are important for species identification, corroborating future studies of phenology and germination. Therefore, the fruits, diaspores and germination of M. ligustroides were morphologically characterized by observations through naked eye, stereoscopic microscopy and scanning electron microscopy. Analyses of chemical composition of seeds were also performed. The fruits are spherical and uniform within the analyzed sample, having a large number of tiny obtriangular yellowish-brown diaspores. The diaspores are considered ananfitropos, and have crystals in their external envelope. Concerning chemical composition, they are proteins. Germination is of phanerocotylar type and starts at 12 Days After Sowing (DAS); at 17 DAS each diaspore may produce up to three seedlings. The morphological characterization performed in this work was efficient to describe the fruits, seeds and germination of this species, thus providing the basis for future studies.

Miconia ligustroides (DC.) Naudim, popularmente conhecida como vassoura-preta ou jacatirão-do-brejo, é amplamente distribuída no Brasil. É uma espécie recomendada para restauração de matas ciliares, e seus frutos são atrativos à fauna. No entanto, pouco se conhece acerca da morfologia de seus frutos e diásporos, e é sabido que estudos de caracterização morfológica são importantes para a identificação de espécies, além de corroborarem estudos futuros de fenologia e germinação. Portanto, os frutos, diásporos e geminação de M. ligustroides foram caracterizados morfologicamente por meio de observações visuais a olho nu, em microscópio estereoscópico e por meio de Microscopia Eletrônica de Varredura. Análises da composição química das sementes também foram realizadas. Os frutos são esféricos e uniformes dentro da amostra analisada. Possuem grande número de diminutos diásporos castanho-amarelados e obtriangulares. Os diásporos são considerados ananfítropos e possuem cristais em seu envoltório externo. Quanto à composição química, são proteicos. A germinação é do tipo fanerocotiledonar e inicia-se aos 12 Dias Após Semeadura (DAS), sendo que aos 17 DAS cada diásporo pode originar até três plântulas. A caracterização morfológica realizada neste trabalho foi eficiente em descrever os frutos, sementes e germinação desta espécie, de modo a subsidiar estudos futuros.

Influência do tratamento ortodôntico com extração no apinhamento da região retromolar / Influence of the orthodontic treatment with extraction on crowding in the mandibular second molar area

Uma das grandes discussões que acompanham a Ortodontia desde os seus primórdios refere-se à dicotomia extrair ou não extrair. Diante de um apinhamento severo, a indicação da extração não causa dúvidas para o ortodontista. Entretanto, diante da protrusão dentária, a decisão pela extração de dentes pode gerar dúvidas. O mesmo acontece em casos limítrofes em pacientes com apinhamento moderado. O presente artigo evoca a avaliação do espaço retromolar nos casos onde existe a dúvida sobre a extração de dentes em um tratamento ortodôntico. Analisada na telerradiografia em norma lateral ou na radiografia panorâmica, a distância entre a distal do primeiro molar e o ramo mandibular pode ser decisiva na opção por extrair. Valorizando a presença dos segundos molares e sua completa diferenciação na cavidade bucal, o ortodontista deve avaliar se o espaço retromolar é suficiente para a irrupção completa dos segundos molares. Em casos onde há falta de espaço na região posterior, o tratamento com extração de dentes e mesialização dos primeiros molares pode favorecer a irrupção dos segundos molares.

One of the controversies regarding orthodontic treatment is related to extraction versus non-extraction treatment. Orthodontists have no doubts that teeth extraction is required to correct severe crowdings. However, the correction of protruded incisors generally raises questions as to whether treatment should be performed with or without extractions. Borderline cases with moderate crowding may also lead to doubts. This article focuses on the evaluation of the mandibular second molar area in cases where the orthodontic treatment includes teeth extraction. The distance between the distal aspect of the mandibular second molar and the mandibular ramus, when analyzed on either lateral or panoramic radiographs, can be decisive as to whether to extract or not. Considering the presence of the second molar and its complete eruption, orthodontists should always evaluate if the mandibular posterior space is sufficient for the eruption of the molars. Therefore, in cases where there is lack of space in the molar area, treatment with teeth extraction and mesial movement of the first molars may facilitate the eruption of the second molars.

Avaliação da morfologia vertebral em pacientes adultos: um indicador maturacional / Evaluation of the vertebral morphology in adult patients: a maturation indicator

A determinação da idade óssea é importante para o diagnóstico e planejamento dos pacientes com má oclusão com comprometimento esquelético e pode ser determinada pelas vértebras cervicais. O objetivo do presente estudo foi avaliar a morfologia das 3ª e 4ª vértebras cervicais em pacientes adultos. A amostra constou de 102 telerradiografias em norma lateral de pacientes selecionados da clínica de Ortodontia da Faculdade de Odontologia - Centro de Pós-Graduação - São Leopoldo Mandic (Unidade Brasília/DF). A morfologia dos corpos das 3ª e 4ª vértebras foi analisada por um único examinador. Diante dos resultados, concluiu-se que o formato vertebral encontrado em pacientes adultos foi o quadrado ou retangular vertical com borda inferior curva.

The determination of bone age is important for diagnosis and planning of patients with skeletal malocclusion with commitment and can be determined by cervical vertebrae. The aim of this study was to evaluate the morphology of the 3rd and 4th cervical vertebrae in adult patients. The sample consisted of 102 lateral radiographs of patients selected from the orthodontic clinic of the Faculty of Dentistry - Graduate Center - St. Leopold Mandic - Unit Brasilia - DF. The morphology of the 3rd and 4th vertebrae was analyzed by a single examiner. The findings show that the square-shaped or, mainly, the rectangular-shaped vertebra with curved lower border was the format founded in adult spine.

Dental age as indicator of adolescence

AIM: The purpose of the present study was to analyze the relationship between root formation of the first premolars and skeletal maturation stages identified in hand-wrist radiographs. METHODS: A cross-sectional study was carried out involving the panoramic and hand-wrist radiographs obtained on the same date of 232 patients, 123 boys and 109 girls aged 4 years and 5 months to 17 years and 12 months. Root formation stages of the first premolars were related to the ossification stages of the sesamoid bone, epiphyseal stages of the phalanx of the thumb and epiphyseal stages of the radius. RESULTS: The studied variables demonstrated statistically significant correlations. CONCLUSION: Roots of the lower first premolars do not reach 2/3 of their complete length before adolescence.

Photographic assessment of nasal morphology following rapid maxillary expansion in children.

OBJECTIVE: The aim of the present study was to use facial analysis to determine the effects of rapid maxillary expansion (RME) on nasal morphology in children in the stages of primary and mixed dentition, with posterior cross-bite. MATERIAL AND METHODS: Facial photographs (front view and profile) of 60 patients in the pre-expansion period, immediate post-expansion period and one year following rapid maxillary expansion with a Haas appliance were evaluated on 2 occasions by 3 experienced orthodontists independently, with a 2-week interval between evaluations. The examiners were instructed to assess nasal morphology and had no knowledge regarding the content of the study. Intraexaminer and interexaminer agreement (assessed using the Kappa statistic) was acceptable. RESULTS: From the analysis of the mode of the examiners' findings, no alterations in nasal morphology occurred regarding the following aspects: dorsum of nose, alar base, nasal width of middle third and nasal base. Alterations were only detected in the nasolabial angle in 1.64% of the patients between the pre-expansion and immediate post-expansion photographs. In 4.92% of the patients between the immediate post-expansion period and 1 year following expansion; and in 6.56% of the patients between the pre-expansion period and one year following expansion. CONCLUSIONS: RME performed on children in stages of primary and mixed dentition did not have any impact on nasal morphology, as assessed using facial analysis.

Programa de extrações seriadas: variáveis relacionadas com a extração de pré-molares / Serial extraction: variables associated to the extraction of premolars

OBJETIVO: verificar o percentual de pacientes que necessitaram extração de dentes permanentes, pré-molares, dentre aqueles tratados com extração de dentes decíduos para correção do apinhamento primário na dentição mista, bem como analisar as possíveis variáveis relacionadas. MÉTODOS: a amostra foi composta por documentações ortodônticas de 70 pacientes na dentição permanente, cujo tratamento iniciou-se na dentição mista com planejamento de um programa de extrações seriadas (PES). Todos os prontuários foram analisados por um único examinador, no intuito de verificar se o PES havia sido cumprido com a extração de dentes permanentes ou se havia sido realizada apenas extração de dentes decíduos. Verificou-se a associação entre a extração de dentes permanentes e as variáveis padrão facial; relação sagital entre as arcadas dentárias; IMPA; proporção tamanho do segundo molar permanente inferior/espaço retromolar; mecânica de controle de espaço e discrepância de modelo (teste exato de Fisher para as variáveis categóricas e modelo de regressão logística para as variáveis numéricas). Os resultados foram considerados para p<0,05. RESULTADOS: dos pacientes que haviam sido tratados com extração de dentes decíduos para a correção do apinhamento na dentição mista, 70 por cento necessitaram de extração de dentes permanentes. A análise estatística não mostrou associação significativa entre as variáveis estudadas e a necessidade de extração de dentes permanentes, com exceção da variável discrepância de modelo. CONCLUSÃO: a discrepância de modelo representou a principal determinante de extração de pré-molares no PES.

OBJECTIVE: To determine the frequency of patients who require extraction of permanent premolars among those treated with extraction of deciduous teeth for the correction of incisor crowding in the mixed dentition and analyze possible associated variables.METHODS: The sample was composed of orthodontic records of 70 patients with permanent dentition whose treatment had begun in the mixed dentition phase and involved serial extraction. All records were analyzed by a single examiner to determine whether serial extraction had been performed with extraction of permanent teeth or only deciduous teeth. Associations were investigated between extraction of permanent teeth and lateral facial pattern, sagittal relationship of the dental arches, incisor-mandibular plane angle, size proportion of mandibular second molar/retromolar space, mechanics for space control and tooth-arch size discrepancy (Fisher's exact test for categorical variables and logistic regression for numeric variables, p<0.05).RESULTS: Among the patients who had been treated with extraction of deciduous teeth, 70% required the extraction of permanent teeth. The statistical analysis revealed no significant associations between the analyzed variables and the need for permanent tooth extraction, with the exception of tooth-arch size discrepancy.CONCLUSION: Tooth-arch size discrepancy was the main factor determining premolar extraction in a serial extraction program.

Photographic assessment of nasal morphology following rapid maxillary expansion in children

OBJECTIVE: The aim of the present study was to use facial analysis to determine the effects of rapid maxillary expansion (RME) on nasal morphology in children in the stages of primary and mixed dentition, with posterior cross-bite. MATERIAL AND METHODS: Facial photographs (front view and profile) of 60 patients in the pre-expansion period, immediate post-expansion period and one year following rapid maxillary expansion with a Haas appliance were evaluated on 2 occasions by 3 experienced orthodontists independently, with a 2-week interval between evaluations. The examiners were instructed to assess nasal morphology and had no knowledge regarding the content of the study. Intraexaminer and interexaminer agreement (assessed using the Kappa statistic) was acceptable. RESULTS: From the analysis of the mode of the examiners' findings, no alterations in nasal morphology occurred regarding the following aspects: dorsum of nose, alar base, nasal width of middle third and nasal base. Alterations were only detected in the nasolabial angle in 1.64 percent of the patients between the pre-expansion and immediate post-expansion photographs. In 4.92 percent of the patients between the immediate post-expansion period and 1 year following expansion; and in 6.56 percent of the patients between the pre-expansion period and one year following expansion. CONCLUSIONS: RME performed on children in stages of primary and mixed dentition did not have any impact on nasal morphology, as assessed using facial analysis.

Ecofisiologia da germinação de sementes de Campomanesia pubescens / Ecophysiology of Campomanesia pubescens seed germination

A gabiroba de arbusto [Campomanesia pubescens (DC.) O. Berg. (Myrtaceae)], espécie nativa do Cerrado é considerada uma planta com potencial melífero, ornamental e medicinal. Com o objetivo de avaliar a influência de diferentes temperaturas, substratos e condições de luminosidade e da secagem, na qualidade fisiológica de suas sementes foram conduzidos dois experimentos. No primeiro utilizou-se sementes com 13 por cento de teor de água, o qual foi realizado em delineamento experimental inteiramente casualizado em esquema fatorial (4x3x2), composto por quatro temperaturas (15-25, 20-30°C alternadas, 25 e 30°C constantes), três substratos (sobre papel, entre areia e entre vermiculita) e duas condições de luminosidade (claro e escuro) e, o segundo com sementes recém extraídas dos frutos e secas superficialmente, contendo 35 por cento de teor de água e após a secagem em estufa com ventilação forçada de ar a temperatura de 30°C por 30, 60, 90, 120, 150, 180, 260, 360, 750 e 810 minutos. Para a avaliação da qualidade fisiológica de sementes de gabiroba de arbusto, a semeadura sobre papel, no escuro e a 30°C possibilita uma melhor expressão do vigor. As sementes de gabiroba de arbusto são intolerantes a secagem, podendo ser classificadas como recalcitrantes.

The gabiroba bush [Campomanesia pubescens (DC.) O. Berg. (Myrtaceae)], a native species of the Cerrado is considered a plant with melliferous, ornamental and medicinal potential. Aiming to evaluate the influence of abiotic factors on seed germination, two experiments were conducted. The first was conducted in a completely randomized design in a factorial design(4x3x2), composed of four temperatures (15-25, 20-30°C alternate, 25 and 30°C constant), 3 substrates (paper, sand and vermiculite) and two light conditions (light and dark). The second experiment aimed to evaluate the tolerance to desiccation of fresh seeds dried superficially, with 35 percent of water, by drying it in oven with forced ventilation of air at 30°C for 30, 60, 90, 120, 150, 180, 260, 360, 750 and 810 minutes. To evaluate the physiological quality of seeds gabiroba, shrub planting on paper, in the dark and 30°C, enables a better expression of the force. The seeds of bush gabiroba are intolerant to desiccation and can be classified as recalcitrant.

Efeito transversal da placa lábio-ativa aberta e fixa associada à extensão lingual de canino decíduo a primeiro molar permanente: estudo em modelos digitais / Transverse effect of the expanded and fixed lip bumper associated to the lingual extension from the deciduous canine to the permanent first molar: a digital model study

Introdução: O objetivo do presente estudo clínico foi avaliar as dimensões transversais do arco dentário inferior com o uso da placa lábio-ativa aberta e fixa associada à extensão lingual de canino decíduo a primeiro molar permanente. Métodos: A amostra constou de 14 pacientes no estágio de dentadura mista e com idade média de oito anos e seis meses. Todos os pacientes realizaram expansão rápida da maxila e expansão lenta do arco inferior com a placa lábio-ativa. Modelos de gesso foram obtidos nas fases de pré-tratamento e pós-tratamento e digitalizados com auxílio do scanner 3Shape R700 3D (3Shape A/S, Copenhagen, Dinamarca). Medições transversais foram realizadas para as distâncias entre as pontas de cúspide dos caninos decíduos, pontas de cúspide mesiovestibulares dos molares decíduos e primeiro molar permanente e pontos médio cervical da face vestibular. O teste t foi utilizado para determinar se o aumento nas larguras transversais foi significativo. Resultados: Observou-se um aumento transversal estatisticamente significativo (p<0,05) que variou de 4,11mm a 4,51mm nos referenciais utilizados no nível das pontas de cúspide. Conclusão: A placa lábio-ativa aberta e fixa apresentou um efeito transversal significativo em aproximadamente seis meses de tratamento.

Introduction: The aim of the current clinical study was to evaluate the transverse dimensions of the lower dental arch with the use of the fixed and expanded lip bumper associated to the lingual extension from the deciduous canine to the permanent first molar. Methods: The sample was comprised of 14 patients in the mixed dentition with a mean age of 8 years 6 months. All patients were submitted to rapid palatal expansion and dentolaveolar expansion of the lower dental arch with a lip bumper. Pre-teatment and post-treatment cast models were obtained and digitized with the 3Shape R700 3D (3Shape A/S, Copenhagen, Denmark) scanner. Transverse distances between the cusp tips of the canines, the mesial buccal cusps of the deciduous molars and of the permanent first molars and between the cervical middle points of the buccal aspects of these teeth were measured. The t tests were performed to determine whether an increase in transverse distances was significant. Results: A statistically significant transverse increase (p<0,05) ranging from 4.11 mm to 4.51 mm was observed in the cusp tips. Conclusion: The fixed and expanded lip bumper presented a significant transverse effect within approximately 6 months of treatment.

Efeito transversal da placa lábio-ativa aberta e fixa associada à extensão lingual de canino decíduo a primeiro molar permanente: estudo em modelos digitais / Transverse effect of the expanded and fixed lip bumper associated to the lingual extension from the deciduous canine to the permanent first molar: a digital model study

Introdução: O objetivo do presente estudo clínico foi avaliar as dimensões transversais do arco dentário inferior com o uso da placa lábio-ativa aberta e fixa associada à extensão lingual de canino decíduo a primeiro molar permanente. Métodos: A amostra constou de 14 pacientes no estágio de dentadura mista e com idade média de oito anos e seis meses. Todos os pacientes realizaram expansão rápida da maxila e expansão lenta do arco inferior com a placa lábio-ativa. Modelos de gesso foram obtidos nas fases de pré-tratamento e pós-tratamento e digitalizados com auxílio do scanner 3Shape R700 3D (3Shape A/S, Copenhagen, Dinamarca). Medições transversais foram realizadas para as distâncias entre as pontas de cúspide dos caninos decíduos, pontas de cúspide mesiovestibulares dos molares decíduos e primeiro molar permanente e pontos médio cervical da face vestibular. O teste t foi utilizado para determinar se o aumento nas larguras transversais foi significativo. Resultados: Observou-se um aumento transversal estatisticamente significativo (p<0,05) que variou de 4,11mm a 4,51mm nos referenciais utilizados no nível das pontas de cúspide. Conclusão: A placa lábio-ativa aberta e fixa apresentou um efeito transversal significativo em aproximadamente seis meses de tratamento

Introduction: The aim of the current clinical study was to evaluate the transverse dimensions of the lower dental arch with the use of the fixed and expanded lip bumper associated to the lingual extension from the deciduous canine to the permanent first molar. Methods: The sample was comprised of 14 patients in the mixed dentition with a mean age of 8 years 6 months. All patients were submitted to rapid palatal expansion and dentolaveolar expansion of the lower dental arch with a lip bumper. Pre-teatment and post-treatment cast models were obtained and digitized with the 3Shape R700 3D (3Shape A/S, Copenhagen, Denmark) scanner. Transverse distances between the cusp tips of the canines, the mesial buccal cusps of the deciduous molars and of the permanent first molars and between the cervical middle points of the buccal aspects of these teeth were measured. The t tests were performed to determine whether an increase in transverse distances was significant. Results: A statistically significant transverse increase (p<0,05) ranging from 4.11 mm to 4.51 mm was observed in the cusp tips. Conclusion: The fixed and expanded lip bumper presented a significant transverse effect within approximately 6 months of treatment